[Sequencing and Phylogenetic Analyses of Structural and Accessory Proteins of Middle East Respiratory Syndrome Coronavirus from the First Imported Case in China, 2015].
Identifieur interne : 001498 ( Main/Exploration ); précédent : 001497; suivant : 001499[Sequencing and Phylogenetic Analyses of Structural and Accessory Proteins of Middle East Respiratory Syndrome Coronavirus from the First Imported Case in China, 2015].
Auteurs : Roujian Lu ; Lirong Zou ; Yanqun Wang ; Yanie Zhao ; Weimin Zhou ; Jie Wu ; Wenling Wang ; Guizhen Wu ; Changwen Ke ; Wenjie TanSource :
- Bing du xue bao = Chinese journal of virology [ 1000-8721 ] ; 2015.
Descripteurs français
- KwdFr :
- Analyse de séquence, Chine, Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie), Génomique, Humains, Infections à coronavirus (transmission), Infections à coronavirus (virologie), Phylogénie, Protéines virales (génétique), Séquence conservée, Évolution moléculaire.
- MESH :
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient, Protéines virales.
- physiologie : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Infections à coronavirus.
- Analyse de séquence, Chine, Génomique, Humains, Infections à coronavirus, Phylogénie, Séquence conservée, Évolution moléculaire.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- China, Conserved Sequence, Coronavirus Infections (transmission), Coronavirus Infections (virology), Evolution, Molecular, Genomics, Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (physiology), Phylogeny, Sequence Analysis, Viral Proteins (genetics).
- MESH :
- chemical , genetics : Viral Proteins.
- geographic : China.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- physiology : Middle East Respiratory Syndrome Coronavirus.
- transmission : Coronavirus Infections.
- virology : Coronavirus Infections.
- Conserved Sequence, Evolution, Molecular, Genomics, Humans, Phylogeny, Sequence Analysis.
Abstract
To investigate the genetic character and origin of the first imported infection case of middle East respiratory syndrome coronavirus (named as MERS-CoV_China GD01), RNA was extracted from swabs of this patient followed by RT-PCR amplification. All coding gene of structural (S, E, M, E) and accessory (ORF3, ORF4a, ORF4b, ORF5, ORF8b) proteins were sequenced and analyzed. Phylogenetic analyses of structural protein coding genes of MERS-CoV_ China GD01 indicates that several substitutes exists in S coding gene and its origin belong group 5 of MERS-CoV, which were recent circulated in Saudi Arabia area, while other three structural genes (N, E, M) were very conserved. Phylogenetic analyses of accessory protein coding genes of MERS-CoV China GD01 indicates that several substitutes exists among ORF3, ORF4a, ORF4b and ORF5, while ORF8b was conserved. In conclusion, genome of MERS-CoV_ China GD01 was general conserved although several genetic variations were found among structural and accessory protein coding genes. This is the first report on sequencing and phylogenetic analyses of the first imported MERS case in China, which may pay the way for prevention and control of imported MERS-CoV infection.
PubMed: 26524904
Affiliations:
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Le document en format XML
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